Overview
- Researchers report measurable transgenic shoot formation across species, including approximately 35% in Nicotiana, 21% in tomato, and 28% in soybean using a modified protocol.
- The approach exploits plants’ wound‑induced regeneration by delivering regulators such as WIND1 and ESR1/IPT via Agrobacterium to induce shoot growth carrying new DNA.
- In soybean, treating germinating seeds followed by about 3.5 weeks in tissue culture produced edited shoots, compared with a conventional timeline of roughly 3–4 months.
- The system integrates with CRISPR tools, reducing dependence on lengthy tissue‑culture steps and potentially broadening access to crop transformation.
- The authors present a proof‑of‑concept that requires optimization for additional crops and further assessment of scalability, biosafety, and regulatory considerations.