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Terasaki Researchers Report Real-Time, Label-Free Platform to Measure Antibody Secretion

The peer-reviewed study details a microfluidic PC-TIR setup that profiles hybridoma secretion within an hour to speed clone selection.

Overview

  • The device integrates a photonic crystal total internal reflection biosensor with a microfluidic microtranswell, concentrating antibodies by placing living hybridoma cells directly above the sensor.
  • The system generates per-cell secretion rates in roughly one hour, enabling rapid comparisons across cell lines.
  • The team reports the platform can pinpoint high‑secreting “powerhouse” clones for earlier selection and process optimization.
  • Findings from the experimental cell study were published on September 11, 2025, in Biosensors and Bioelectronics (DOI: 10.1016/j.bios.2025.117979).
  • Researchers present the approach as a path to more efficient, lower‑cost monoclonal antibody manufacturing, though industrial-scale validation has not yet been shown.