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Study Ties Burst of Gene Isoforms to the Dawn of Vertebrates

Long-read sequencing across sea squirt, lamprey and frog pinpoints expanded diversity in transcription-factor effectors of cell-signaling pathways.

Overview

  • University of St Andrews researchers report a marked rise in transcript isoforms per gene at the invertebrate-to-vertebrate transition, published in BMC Biology (2026).
  • This increase is concentrated in transcription factor effectors at the ends of key intercellular signaling pathways that govern developmental decisions.
  • New long-read datasets from a sea squirt (invertebrate), a lamprey (early vertebrate) and a frog resolved full-length transcripts to quantify isoform diversity with greater precision.
  • The pattern suggests vertebrate complexity expanded by diversifying outputs of existing signaling genes rather than broadly adding new genes.
  • Authors highlight the need for functional assays to test isoform roles and note potential implications for precision therapies targeting pathways often involved in disease.