Overview
- The Van Andel Institute introduced scDEEP-mC in Nature Communications on July 8, making the protocol publicly available.
- The method produces high-resolution, genome-wide, allele-resolved maps of DNA methylation in individual cells without averaging signals across cell populations.
- scDEEP-mC supports analyses of hemimethylation, cellular age estimation via epigenetic clocks, and whole-chromosome X-inactivation profiling.
- Its unique design allows observation of methylation patterns at distinct stages of DNA replication within single cells.
- Early adopters are applying scDEEP-mC to identify rare cell subtypes and pinpoint early epigenetic alterations linked to cancer initiation.