The fixture kicks off at 21:10 at Estadio Único Madre de Ciudades in Santiago del Estero with live coverage on TyC Sports and streaming via TyC Sports Play. Marcelo Gallardo’s squad will be without forward Maximiliano Salas after he suffered a knee sprain in training. Mariano Campodónico takes charge of San Martín de Tucumán for the first time after the club replaced Ariel Martos following uneven league form. The winner will meet Unión de Santa Fe in the Round of 16 after Santa Fe defeated Rosario Central in the previous round. River and San Martín last met on February 24, 2019, when River won 2–1 away thanks to goals from Juan Fernando Quintero and Matías Suárez.