Overview
- CLASSIC enables the simultaneous creation and analysis of hundreds of thousands to millions of genetic circuit designs.
- Long-read sequencing captures full circuit architectures while short-read sequencing provides precise barcoding to tag and track each design.
- Engineered human embryonic kidney cells with fluorescence readouts were used to measure circuit activity and map sequence to function.
- Machine-learning models trained on the large datasets accurately predicted performance for untested designs, with 40 of 40 manual checks matching.
- Researchers say the platform could accelerate development of cell-based therapies and other synthetic biology applications.