Overview

• Researchers identified LINC01235 via RNA sequencing of human TNBC organoids and TCGA data analysis, revealing a correlation with NFIB expression.
• CRISPR-mediated knockout and antisense knockdown of LINC01235 lowered NFIB levels and suppressed the formation of TNBC tumor organoids.
• Functional assays confirmed that LINC01235 positively regulates NFIB transcription to modulate NOTCH signaling and drive TNBC cell proliferation.
• Triple-negative breast cancer makes up 10–15% of breast cancers, disproportionately affecting younger and African American women and lacking effective targeted therapies.
• The research team plans to validate additional lncRNA targets and advance preclinical development of lncRNA-based interventions for aggressive breast cancer.