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PFAS Mixture at Real-World Levels Alters Male Mice’s Sperm and Embryo Gene Activity

Researchers modeled a Williamtown groundwater mix to test real-world reproductive risks in mice.

Overview

  • A University of Newcastle study in Communications Biology exposed male mice for 12 weeks to a PFAS cocktail matching concentrations from a Williamtown groundwater monitoring well, with an additional 10-times higher dose group.
  • Daily sperm production fell during exposure, and key male hormones testosterone and dihydrotestosterone were reduced at environmentally relevant levels.
  • Despite normal motility and fertilising capacity in lab tests, sperm carried altered gene-regulating molecules linked to disrupted gene expression in early embryos from unexposed females.
  • Some hormonal changes appeared only in the lower, Williamtown-matched dose, consistent with non-linear responses seen with endocrine-disrupting chemicals.
  • Authors say the pre-clinical findings warrant further investigation for communities with PFAS contamination, aligning with broader monitoring that shows widespread Australian exposure and growing ecological concerns.