Overview

• The nuclear morphometric pipeline uses AI analysis of high-resolution images and standard nuclear stains to assign a senescence score from –20 to +20
• Validation in mouse models showed the tool accurately distinguishes healthy and senescent cells across ages ranging from three months to over two years
• In injured muscle, the pipeline tracked dynamic shifts in senescent and non-senescent stem, endothelial, and immune cells, and revealed a tenfold rise in senescent cartilage cells in arthritic mice
• NYU has filed a patent application for the NMP and intends to make the platform accessible to other researchers
• The research team plans further experiments to test the pipeline on human tissue samples and to integrate it with additional biomarker assays