Overview

• The cubosome approach mixes fusogenic lipid nanoparticles with exosomes at room temperature to encapsulate large biomolecules like mRNA in under 10 minutes without specialized equipment.
• The technique achieved over 98% encapsulation efficiency while preserving exosome structural integrity and biological activity.
• Engineered hybrid exosomes crossed the blood–brain barrier in preclinical models and exhibited a ‘homing’ effect that directs them back to their cell of origin.
• This mix-and-go strategy bypasses damaging electroporation and complex purification steps that have hindered earlier exosome loading methods.
• Researchers are now conducting safety evaluations and developing mass-production protocols to support upcoming clinical translation.