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Lab-Defined Microbial Community Recreates Fine Chocolate Flavor

Peer-reviewed research identifies pH, temperature, microbe mix as the main levers in cocoa fermentation.

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Overview

  • The Nature Microbiology paper links specific pH and temperature trajectories with distinct microbial communities to the chemistry that underpins fine cocoa aroma.
  • Researchers sampled on-farm fermentations in Colombia’s Santander, Huila, and Antioquia, controlling for bean genetics to isolate fermentation effects.
  • A curated nine-species mix of bacteria and yeasts, including fungal genera such as Torulaspora and Saccharomyces, reproduced fine-flavour profiles in sterile lab fermentations, confirmed by blind sensory panels.
  • Tasters rated lab, Santander, and Huila liquors as fine-flavour while Antioquia resembled bulk cocoa, mirroring measured differences in microbes and fermentation dynamics.
  • The team proposes starter cultures to standardize quality and enable new profiles, with broader field trials needed to assess performance across regions and climates.