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ISS Study Finds Microgravity Reshapes Phage–Bacteria Evolution, Boosting Activity Against Drug-Resistant E. coli

The PLOS Biology paper from University of Wisconsin–Madison identifies spaceflight as a discovery environment for engineering phages with improved efficacy.

Overview

  • Parallel experiments on the International Space Station and on Earth showed that T7 phage infection of E. coli was initially delayed in microgravity before proceeding.
  • Whole-genome sequencing revealed distinct mutation patterns in both phages and bacteria in space compared with terrestrial controls.
  • Deep mutational scanning of the phage receptor binding protein showed a different mutational landscape in microgravity that produced variants with superior activity against some T7‑resistant, UTI‑causing E. coli on Earth.
  • Researchers cite altered fluid mixing and encounter rates in near‑weightless conditions as a likely driver of the early infection slowdown.
  • The team emphasizes ISS constraints—small sample sizes, fixed hardware, and freezing or long storage—and calls for follow‑up studies to refine phage engineering and inform astronaut health.