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DNA Study Finds Paratyphoid and Relapsing Fever in Napoleon’s 1812 Dead

Metagenomic sequencing of teeth from a Vilnius mass grave identifies pathogens that broaden the understanding of disease during the retreat.

Overview

  • In a Current Biology study, four of 13 sampled soldiers carried Salmonella enterica Paratyphi C, consistent with paratyphoid fever, and two carried Borrelia recurrentis, which causes louse-borne relapsing fever.
  • Researchers did not detect the typhus bacterium Rickettsia prowazekii in these 13 individuals, though earlier work on different remains from the site found evidence of typhus and trench fever.
  • The team used unbiased shotgun sequencing of dental pulp to recover ancient pathogen DNA, enabling detection beyond targets of older PCR-era tests.
  • The remains came from a Vilnius, Lithuania mass grave discovered in 2001 that holds roughly 2,000 to 3,000 soldiers from Napoleon’s retreat.
  • Authors caution that the sample is small and localized and call for larger, geographically broader surveys to map the full disease landscape of the 1812 campaign.