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Defined Microbial Cultures Reproduce Fine-Flavor Chocolate Attributes in Lab Study

The University of Nottingham team used field data from Colombian farms to assemble a nine-microbe community that replicated fine-flavor profiles in lab ferments, setting the stage for controlled starter cultures yet leaving on-farm adoption untested.

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Overview

  • Field sampling in Santander, Huila and Antioquia mapped temperature, pH and native microbial dynamics that correlate with distinct flavor outcomes in spontaneous ferments.
  • Sequencing and metabolic analysis identified nine key microbial species whose combined activity predicts floral, fruity and citrus notes in fine-flavor cocoa.
  • Lab fermentations of sterile cocoa beans using the defined community reproduced target aroma compounds and delivered reduced bitterness compared with uncontrolled processes.
  • The study, published today in Nature Microbiology, proposes that starter cultures could standardize and expand flavor control in chocolate production, mirroring advances in beer and cheese.
  • Researchers highlight that validating the defined cultures at farm scale, securing farmer uptake and addressing socio-environmental challenges remain critical next steps.