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Cincinnati Children’s and Roche Develop Human Liver Organoid Microarray to Predict Immune-Linked Drug Toxicity

The platform pairs iPSC-derived liver tissues with each donor’s CD8+ T cells to capture patient-specific immune responses.

Overview

  • The peer-reviewed findings were published online on Sept. 26, 2025, in Advanced Science.
  • As a proof of concept, the model reproduced flucloxacillin-triggered liver injury in HLA-B*57:01 carriers, including T cell activation, cytokine release, and hepatocyte damage.
  • Researchers aim to close a known gap in preclinical testing where standard assays and animal models miss idiosyncratic, immune-mediated liver injury.
  • The CuSTOM Accelerator is advancing automation and high-throughput screening to test larger, genetically diverse donor cohorts.
  • The project involved collaborators from Genentech and Molecular Devices, with funding from Roche, Danaher, and the Farmer Family Foundation.