Overview
- Researchers from Stanford University and Stockholm University report the findings in Cell on November 26, 2025 (DOI: 10.1016/j.cell.2025.11.008).
- KLF1, a transcription factor essential for red blood cell development, was quantitatively profiled for how it recognizes DNA.
- In vitro affinity measurements were shown to be consistent with KLF1’s binding behavior observed in human cells, enabling a quantitative framework for in-cell binding.
- The study finds that KLF1 senses a broader surrounding DNA sequence than previously recognized, reshaping views of transcription factor specificity.
- The team used high-throughput assays in test tubes by imaging DNA sequencing chips and in cells via a methylation-based readout, with implications for interpreting disease-linked noncoding variants.