Overview

• Using Cell Painting microscopy, researchers screened 244 flavonoids, identifying six that selectively kill bladder cancer cells without harming normal cells.
• The SPACe computational tool enabled efficient analysis of large-scale phenotypic data on standard desktop computers, making high-throughput screening more accessible.
• Three flavonoids reduced tumor growth in advanced 3D culture models, including spheroids and chorioallantoic membrane systems, confirming their potential efficacy.
• Xanthohumol, derived from hops, was linked to reduced lipid metabolism in bladder cancer cells, highlighting a novel mechanism of action.
• The team plans to evaluate the safety and efficacy of these flavonoids in animal models as a step toward developing new cancer therapies.