Overview
- Working in H1 embryonic stem cells and HFFc6 fibroblasts, the consortium delivers the most comprehensive 3D and time-resolved genome maps reported to date.
- Researchers cataloged 141,365 regulatory loops in stem cells and 146,140 in fibroblasts, detailing loop anchors linked to gene regulation.
- Data from complementary assays were merged into an Integrative Genome Modeling platform that generated about 1,000 single-cell 3D genome models capturing structural variability.
- The team benchmarked mapping techniques and produced classifications of chromosomal domains along with their positions inside the nucleus.
- Deep-learning tools trained on the integrated datasets predict 3D folding from DNA sequence, enabling in silico assessment of non-coding variants and pointing to future disease-focused applications.